Proof of concept

Mucosa Testing Vitellogenin

Proof of concept: Protein chemistry

Serum MALDI-TOF analysis of the 140 kDa band of goldfish exposed to 500 ng/L E2

Peptide mass fingerprinting against NCBI non-redundant database

a Search engine: MASCOT; enzyme: trypsin.

Proof of concept: Immunology

Western blot membranes of the samples of C. carpio (a, b), D. rerio (a, c), O. latipes (a, b), P. promelas (a – c) and C. auratus (a, c), and from O. niloticus (d).

Densiogram of serum, tail homogenate and epidermal mucosa of the goldfish exposed to 17ß-estradiol

Proof of concept: Molecular biology

(A) Estrogen receptors
(B) Goldfish vtg
(C) Carp vtg
(D) Zebrafish vtg
(E) Bluegill vtg (and tilapia vtg gene)
(F) Tilapia vtg
(G) Medaka vtg
(H) Fathead minnow vtg

Evaluation of sensitive enzyme-linked immunosorbent assays

Your article “Kinetic determination of vitellogenin induction in the epidermis of cyprinid and perciform fishes: Evaluation of sensitive enzyme-linked immunosorbent assays (ELISA)” was among the top 10 nominated articles based on scientific impact, public and professional interest, comprehension, and experimental design and quality

E-Mail on behalf of G. Allen Burton, Jr. Editor-in-Chief. Environmental Toxicology & Chemistry, from March 7th 2017 to the authors